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KMID : 0829220110350020067
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Korean Journal of Oral and Maxillofacial Pathology
2011 Volume.35 No. 2 p.67 ~ p.75
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A Study of the Expression of OPG and RANKL due to Titanium Particles in Periodontal Ligament Cells
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Cho Hyung-Jun
Na Yu-Jin
Jeong Yong-Suk
Hong Ji-Suk
Kim Yoon-Ji
Lee Chong-Heon
Cheon Gae-Sik
Park Yang-Ho
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Abstract
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The purpose of this study was to investigate the effect of cytokine-induced osteoclastogenesis on tooth movement related to orthodontic force. We evaluated the cytotoxicity as well as the expression of OPG and RANKL, which influence the homeostasis of bone metabolism. Titanium particles were applied to human periodontal ligament cells and subcultured fourth generation cells. The ALP assay and the MTT assay were used to assess changes in cytotoxicity. After 48 hours, cytotoxicity increased proportionally with the concentration of titanium. With 20 ㎎, the cytotoxicity was the lowest. RT-PCR was used for assessing mRNA levels of OPG and RANKL; after 96 hours, the mRNAs of OPG and RANKL increased steeply. A western blot analysis showed that with 20 ㎎ of titanium, the protein expression of OPG increased linearly with time, especially after 96 hours, while the protein expression of RANKL did not show significant changes with titanium processing. Given the increase in OPG expression after the initial cytotoxicity, changes in cytotoxicity with titanium may be attributable to the antagonistic effect of OPG on cytotoxicity.
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KEYWORD
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OPG, Peridontal ligament cells, RANKL, Titanium particles
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